Please use this identifier to cite or link to this item: http://hdl.handle.net/10637/699

Determination of alpha-tocopherol and alpha-tocopheryl acetate in diets of experimental animals : study of stability in the diets.

Title: Determination of alpha-tocopherol and alpha-tocopheryl acetate in diets of experimental animals : study of stability in the diets.
Authors : Rupérez Pascualena, Francisco Javier
Barbas Arribas, Coral.
Castro, Mario
Herrera Castillón, Emilio.
Keywords: Oils.Food analysis.Tocopherol.Tocoplleryl acetate.Vitamins.Phenyldodecane.
Abstract: A simple method is described which permits, avoiding saponification, a-tocopherol and cx-to:opheryl acetate measurement in semi-synthetic diets for experimental ao.imals by HPLC, with both UV and fluoresccn..:e detection. Phenyldodecane was chosen as internal standard with remarkable performances, an<l EDTA and BHT were added to prevent oxidation in aqueous and non-aqueous phases respectively. The mobile phase was methanol-water (94:6, v/v) at a flow-rate of 2 ml/min. Samples were homogenized and extracted twice with n-hexane by probe sonication. Extracts were evaporated to dryness and redissolved with chloroform-methanol (1:1, v/v). validation parameters were studied between 25 ng and 6 µ,g for a-tocopherol and between 3 and 24.2 µ,g for a-tocopheryl acetate, which corresponds to the range of values in the existing diets. Results had correlation coefficients >0.99; recoveries >85%; R.S.D.<6%, so the method :s adequate to control vitamin E intake in animals as well as vitamin E stability in food during storage.
Description: En: Journal of chromatografy A. ISSN 0021-9673 1999. n. 839 : 93-99 p.
URI: http://hdl.handle.net/10637/699
Rights : http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
Issue Date: 19-Sep-1997
Center : Universidad San Pablo-CEU
Appears in Collections:Facultad de Farmacia





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