Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10637/699

Determination of alpha-tocopherol and alpha-tocopheryl acetate in diets of experimental animals : study of stability in the diets.

Título : Determination of alpha-tocopherol and alpha-tocopheryl acetate in diets of experimental animals : study of stability in the diets.
Autor : Rupérez Pascualena, Francisco Javier
Barbas Arribas, Coral.
Castro, Mario
Herrera Castillón, Emilio.
Materias: Oils.Food analysis.Tocopherol.Tocoplleryl acetate.Vitamins.Phenyldodecane.
Resumen : A simple method is described which permits, avoiding saponification, a-tocopherol and cx-to:opheryl acetate measurement in semi-synthetic diets for experimental ao.imals by HPLC, with both UV and fluoresccn..:e detection. Phenyldodecane was chosen as internal standard with remarkable performances, an<l EDTA and BHT were added to prevent oxidation in aqueous and non-aqueous phases respectively. The mobile phase was methanol-water (94:6, v/v) at a flow-rate of 2 ml/min. Samples were homogenized and extracted twice with n-hexane by probe sonication. Extracts were evaporated to dryness and redissolved with chloroform-methanol (1:1, v/v). validation parameters were studied between 25 ng and 6 µ,g for a-tocopherol and between 3 and 24.2 µ,g for a-tocopheryl acetate, which corresponds to the range of values in the existing diets. Results had correlation coefficients >0.99; recoveries >85%; R.S.D.<6%, so the method :s adequate to control vitamin E intake in animals as well as vitamin E stability in food during storage.
Descripción : En: Journal of chromatografy A. ISSN 0021-9673 1999. n. 839 : 93-99 p.
URI : http://hdl.handle.net/10637/699
Derechos: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
Fecha de publicación : 19-sep-1997
Centro : Universidad San Pablo-CEU
Aparece en las colecciones: Facultad de Farmacia





Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.