A multiplatform metabolomic approach to the basis of antimonial action and resistance in Leishmania infantum

Abstract

Abstract: The goal of this work is to test whether a metabolomic platform, gathering different complementary analytical techniques, will improve the metabolite coverage achieved by single separation techniques, applied to the mechanism of action and resistance to Sb3+ in Leishmania infantum. This was accomplished first through the untargeted analysis of metabolic snapshots of treated and untreated parasites both resistant and responders, utilizing a multiplatform approach to give the widest as possible coverage of the metabolome, and additionally through novel monitoring of the origin of the detected alterations through a 13C traceability experiment.

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Resumen: El objetivo de este trabajo es comprobar los mecanismos de acción y resistencia a Sb3+ en Leishmania infantum desde un enfoque multiplataforma con técnicas analíticas complementarias y conseguir una mejora en la cobertura metabólica global. Primeramente se analizaron los perfiles metabólicos en parásitos tratados, no tratados y resistentes. Obtenidas las pertinentes conclusiones, se procedió a la monitorización del origen de las alteraciones metabólicas detectadas a través de una novedosa metodología para la trazabilidad de 13C.