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http://hdl.handle.net/10637/15168
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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.other | Universidad San Pablo-CEU. Facultad de Farmacia. Departamento de Química y Bioquímica | - |
dc.contributor.other | Grupo de Metabolismo y Función Vascular (MET-VASC) | - |
dc.creator | Rodríguez, Carlos M. | - |
dc.creator | Velásquez-Berrio, Manuela | - |
dc.creator | Rúa, Carolina | - |
dc.creator | Viana Arribas, Marta | - |
dc.creator | Abrahams, Vikki M. | - |
dc.creator | Cadavid, Ángela P. | - |
dc.creator | Álvarez, Ángela M. | - |
dc.date.accessioned | 2024-01-26T17:21:10Z | - |
dc.date.available | 2024-01-26T17:21:10Z | - |
dc.date.issued | 2021-11-29 | - |
dc.identifier.citation | Rodríguez CM, Velásquez-Berrío M, Rúa C, Viana M, Abrahams VM, Cadavid AP and Alvarez AM (2021) Antiphospholipid Antibodies From Women With Pregnancy Morbidity and Vascular Thrombosis Induce Endothelial Mitochondrial Dysfunction, mTOR Activation, and Autophagy. Front. Physiol. 12:706743. doi: 10.3389/fphys.2021.706743 | - |
dc.identifier.issn | 1664-042X | - |
dc.identifier.uri | http://hdl.handle.net/10637/15168 | - |
dc.description.abstract | Antiphospholipid syndrome (APS) is an autoimmune disease characterized by thrombosis and pregnancy morbidity (PM) obstetric events together with persistent high titers of circulating antiphospholipid antibodies (aPL). Several mechanisms that explain the development of thrombosis and PM in APS include the association of aPL with alterations in the coagulation cascade and inflammatory events. Other mechanisms disturbing cellular homeostases, such as mitochondrial dysfunction, autophagy, and cell proliferation, have been described in other autoimmune diseases. Therefore, the objective of this study was to investigate the impact of aPL from different patient populations on endothelial cell mitochondrial function, activation of the mammalian target of rapamycin (mTOR) and autophagy pathways, and cellular growth. Using an in vitro model, human umbilical vein endothelial cells (HUVECs) were treated with polyclonal immunoglobulin G (IgG) purified from the serum of women with both PM and vascular thrombosis (PM/VT), with VT only (VT), or with PM and non-criteria aPL (seronegative-obstetric APS, SN-OAPS). We included IgG from women with PM without aPL (PM/aPL-) and healthy women with previous uncomplicated pregnancies (normal human serum, NHS) as control groups. Mitochondrial function, mTOR activation, autophagy, and cell proliferation were evaluated by Western blotting, flow cytometry, and functional assays. IgG from women with PM/VT increased HUVEC mitochondrial hyperpolarization and activation of the mTOR and autophagic pathways, while IgG from patients with VT induced endothelial autophagy and cell proliferation in the absence of elevated mTOR activity or mitochondrial dysfunction. IgG from the SN-OAPS patient group had no effect on any of these HUVEC responses. In conclusion, aPL from women with PM and vascular events induce cellular stress evidenced by mitochondrial hyperpolarization and increased activation of the mTOR and autophagic pathways which may play a role in the pathogenesis of obstetric APS. | en_EN |
dc.language.iso | en | - |
dc.publisher | Frontiers Media | - |
dc.relation.ispartof | Frontiers in Physiology | - |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es | - |
dc.rights | Open Access | - |
dc.subject | Antiphospholipid antibodies | en_EN |
dc.subject | Antiphospholipid syndrome | en_EN |
dc.subject | Endothelial cell | en_EN |
dc.subject | Mitochondria | en_EN |
dc.subject | mTOR | en_EN |
dc.subject | Autophagy | en_EN |
dc.title | Antiphospholipid Antibodies From Women With Pregnancy Morbidity and Vascular Thrombosis Induce Endothelial Mitochondrial Dysfunction, mTOR Activation, and Autophagy | en_EN |
dc.type | Artículo | - |
dc.relation.projectID | Committee for Research Development (CODI, Universidad de Antioquia UdeA), Grant number 2015-7448 | - |
dc.relation.projectID | MV-B was supported by a fellowship from MINCIENCIAS (Grant number 757 from 2016) | - |
dc.centro | Universidad San Pablo-CEU | - |
Aparece en las colecciones: | Facultad de Farmacia |
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