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Double specific nested PCR and diagnostic SNP assay for species identification in lynx fecal critical samples


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Título : Double specific nested PCR and diagnostic SNP assay for species identification in lynx fecal critical samples
Autor : Cruz Varona, Alejandra
Matallanas, Beatriz
Lobón-Rovira, Javier
Casanovas, Jorge G.
Alonso, Germán
Arana, Pilar
Materias: Iberian LynxFecal DNAThreatened speciesNested PCRSNP
Editorial : Springer
Citación : Alejandra Cruz Varona; Beatriz Matallanas Peñas; Javier Lobón Rovira; Jorge García Casanovas; Germán Alonso Campos; Pilar de Arana Montes (2019) Double specific nested PCR and diagnostic SNP assay for species identification in lynx fecal critical samples. Conservation Genetics Resources 11 (2): 173-175 https://doi.org/10.1007/s12686-018-0993-4 , Springer Netherlands, Jun 2019. Online: 16/01/2018. ISSN 1877-7260.
Resumen : The Iberian lynx – Lynx pardinus (Temmink, 1827) – is one of the most threatened felines in the world so, any biological indication of its presence is significant. However, it is a very elusive species and physical evidence through direct visualization or camera trapping is difficult to obtain. Genetic noninvasive methods, in particular, molecular scatology, are a reliable alternative to unequivocally identify the presence of a species in a given geographical area. We have developed a highly specific and sensitive molecular method to identify the presence of a critically endangered lynx species from severely deteriorated fecal samples. The method consists in a double specific nested PCR of mitochondrial D-loop sequences and two diagnostic SNPs detected by a multiplex primer extension reaction.
URI : http://hdl.handle.net/10637/15167
Derechos: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
ISSN : 1877-7260
Fecha de publicación : 19-ene-2018
Centro : Universidad San Pablo-CEU.
Aparece en las colecciones: Medicina





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