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Pathogenesis of intradermal staphylococcal infections: rabbit experimental approach to natural "Staphylococcus aureus" skin infections


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Título : Pathogenesis of intradermal staphylococcal infections: rabbit experimental approach to natural "Staphylococcus aureus" skin infections
Autor : Muñoz Silvestre, Asunción
Penadés Fons, Mariola
Selva Martínez, Laura
Pérez Fuentes, Sara
Moreno Grúa, Elena
García Quirós, Ana
Pascual, Juan José
Arnau Bonachera, Alberto
Barragán Hernández, Agustín
Corpa Arenas, Juan Manuel
Viana Martín, David
Materias: EstafilococosStaphylococcusEnfermedad de la pielSkin diseasesEnsayoTestingConejosRabbits
Editorial : Elsevier
Citación : Muñoz-Silvestre, A., Penadés, M., Selva, L., Pérez-Fuentes, S., Moreno-Grua, E., García-Quirós, A., Pascual, J.J., Arnau-Bonachera, A., Barragán, A., Corpa, J.M. & Viana, D. (2020). Pathogenesis of intradermal staphylococcal infections: rabbit experimental approach to natural "Staphylococcus aureus" skin infections. The American Journal of Pathology, vol. 190, i. 6 (jun.), pp. 1188-1210. DOI: https://doi.org/10.1016/j.ajpath.2020.01.019
Resumen : Despite the enormous efforts made to achieve effective tools that fight against Staphylococcus aureus, the results have not been successful. This failure may be due to the absence of truly representative experimental models. To overcome this deficiency, the present work describes and immunologically characterizes the infection for 28 days, in an experimental low-dose (300 colony-forming units) intradermal model of infection in rabbits, which reproduces the characteristic staphylococcal abscess. Surprisingly, when mutant strains in the genes involved in virulence (JΔagr, JΔcoaΔvwb, JΔhla, and JΔpsmα) were inoculated, no strong effect on the severity of lesions was observed, unlike other models that use high doses of bacteria. The inoculation of a human rabbitized (FdltBr) strain demonstrated its capacity to generate a similar inflammatory response to a wild-type rabbit strain and, therefore, validated this model for conducting these experimental studies with human strains. To conclude, this model proved reproducible and may be an option of choice to check both wild-type and mutant strains of different origins.
Descripción : Este es el post-print del siguiente artículo: Muñoz-Silvestre, A., Penadés, M., Selva, L., Pérez-Fuentes, S., Moreno-Grua, E., García-Quirós, A., Pascual, J.J., Arnau-Bonachera, A., Barragán, A., Corpa, J.M. & Viana, D. (2020). Pathogenesis of intradermal staphylococcal infections: rabbit experimental approach to natural "Staphylococcus aureus" skin infections. The American Journal of Pathology, vol. 190, i. 6 (jun.), pp. 1188-1210, que se ha publicado de forma definitiva en: https://doi.org/10.1016/j.ajpath.2020.01.019
This is the peer reviewed version of the following article: Muñoz-Silvestre, A., Penadés, M., Selva, L., Pérez-Fuentes, S., Moreno-Grua, E., García-Quirós, A., Pascual, J.J., Arnau-Bonachera, A., Barragán, A., Corpa, J.M. & Viana, D. (2020). Pathogenesis of intradermal staphylococcal infections: rabbit experimental approach to natural "Staphylococcus aureus" skin infections. The American Journal of Pathology, vol. 190, i. 6 (jun.), pp. 1188-1210, which has been published in final form at: https://doi.org/10.1016/j.ajpath.2020.01.019
URI : http://hdl.handle.net/10637/15158
Derechos: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
ISSN : 0002-9440
1525-2191 (Electrónico)
Fecha de publicación : jun-2020
Centro : Universidad Cardenal Herrera-CEU
Aparece en las colecciones: Dpto. Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos





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