Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10637/15087

A SARS-CoV-2 full genome sequence of the B.1.1 lineage sheds light on viral evolution in Sicily in late 2020


Vista previa

Ver/Abrir:
 SARS_Padilla_FIPH_2023.pdf
1 MB
Adobe PDF
Título : A SARS-CoV-2 full genome sequence of the B.1.1 lineage sheds light on viral evolution in Sicily in late 2020
Autor : Padilla Blanco, Miguel
Gucciardi, Francesca
Rubio, Vicente
Lastra, Antonio
Lorenzo Bermejo, Teresa
Ballester Llobell, Beatriz
González Pastor, Andrea
Veses Jiménez, Verónica.
Macaluso, Giusi
Sheth Shah, Chirag Chandrakant
Pascual Ortiz, Marina
Maiques Fernández, Elisa
Rubio Guerri, Consuelo
Purpari, Giuseppa
Guercio, Annalisa
Materias: VirusVirusesGenomaGenomeSARS-CoV-2 (Virus)ItaliaItaly
Editorial : Frontiers Media
Citación : Padilla-Blanco, M., Gucciardi, F., Rubio, V., Lastra, A., Lorenzo, T., Ballester, B., González-Pastor, A., Veses, V., Macaluso, G., Sheth, C.C., Pascual-Ortiz, M., Maiques, E., Rubio-Guerri, C., Purpari, G. & Guercio, A. (2023). A SARS-CoV-2 full genome sequence of the B.1.1 lineage sheds light on viral evolution in Sicily in late 2020. Frontiers in Public Health, vol. 11, art. 1098965 (26 jan.). DOI: https://doi.org/10.3389/fpubh.2023.1098965
Resumen : To investigate the influence of geographic constrains to mobility on SARS-CoV-2 circulation before the advent of vaccination, we recently characterized the occurrence in Sicily of viral lineages in the second pandemic wave (September to December 2020). Our data revealed wide prevalence of the then widespread through Europe B.1.177 variant, although some viral samples could not be classified with the limited Sanger sequencing tools used. A particularly interesting sample could not be fitted to a major variant then circulating in Europe and has been subjected here to full genome sequencing in an attempt to clarify its origin, lineage and relations with the seven full genome sequences deposited for that period in Sicily, hoping to provide clues on viral evolution. The obtained genome is unique (not present in databases). It hosts 20 single-base substitutions relative to the original Wuhan-Hu-1 sequence, 8 of them synonymous and the other 12 encoding 11 amino acid substitutions, all of them already reported one by one. They include four highly prevalent substitutions, NSP12:P323L, S:D614G, and N:R203K/G204R; the much less prevalent S:G181V, ORF3a:G49V and N:R209I changes; and the very rare mutations NSP3:L761I, NSP6:S106F, NSP8:S41F and NSP14:Y447H. GISAID labeled this genome as B.1.1 lineage, a lineage that appeared early on in the pandemic. Phylogenetic analysis also confirmed this lineage diagnosis. Comparison with the seven genome sequences deposited in late 2020 from Sicily revealed branching leading to B.1.177 in one branch and to Alpha in the other branch, and suggested a local origin for the S:G118V mutation.
URI : http://hdl.handle.net/10637/15087
Derechos: https://creativecommons.org/licenses/by/4.0/deed.es
Open Access
ISSN : 2296-2565 (Electrónico)
Fecha de publicación : 26-ene-2023
Centro : Universidad Cardenal Herrera-CEU
Aparece en las colecciones: Dpto. Ciencias Biomédicas





Los ítems de DSpace están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.