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Celecoxib Microparticles for Inhalation in COVID-19-Related Acute Respiratory Distress Syndrome


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Título : Celecoxib Microparticles for Inhalation in COVID-19-Related Acute Respiratory Distress Syndrome
Autor : Villa Hermosilla, Mónica Carolina
Negro, Sofía
Barcia, Emilia
Hurtado Marcos, Carolina
Montejo Rubio, María Consuelo
Alonso, María
Fernandez-Carballido, Ana
Materias: CelecoxibCOVID-19PLGAMicroparticlesMacrophagesInhalation
Editorial : MDPI
Citación : Villa-Hermosilla, M.-C.; Negro, S.; Barcia, E.; Hurtado, C.; Montejo, C.; Alonso, M.; Fernández-Carballido, A. Celecoxib Microparticles for Inhalation in COVID-19-Related Acute Respiratory Distress Syndrome. Pharmaceutics 2022, 14, 1392. https://doi.org/10.3390/ pharmaceutics14071392
Resumen : Inhalation therapy is gaining increasing attention for the delivery of drugs destined to treat respiratory disorders associated with cytokine storms, such as COVID-19. The pathogenesis of COVID-19 includes an inflammatory storm with the release of cytokines from macrophages, which may be treated with anti-inflammatory drugs as celecoxib (CXB). For this, CXB-loaded PLGA microparticles (MPs) for inhaled therapy and that are able to be internalized by alveolar macrophages, were developed. MPs were prepared with 5% and 10% initial percentages of CXB (MP-C1 and MPC2). For both systems, the mean particle size was around 5 μm, which was adequate for macrophage uptake, and the mean encapsulation efficiency was >89%. The in vitro release of CXB was prolonged for more than 40 and 70 days, respectively. The uptake of fluorescein-loaded PLGA MPs by the RAW 264.7 macrophage cell line was evidenced by flow cytometry, fluorescence microscopy and confocal microscopy. CXB-loaded PLGA MPs did not produce cytotoxicity at the concentrations assayed. The anti-inflammatory activity of CXB (encapsulated and in solution) was evaluated by determining the IL-1, IL-6 and TNF-α levels at 24 h and 72 h in RAW 264.7 macrophages, resulting in a higher degree of reduction in the expression of inflammatory mediators for CXB in solution. A potent degree of gene expression reduction was obtained with the developed CXB-loaded MPs.
URI : http://hdl.handle.net/10637/14768
Derechos: http://creativecommons.org/licenses/by/4.0/deed.es
OpenAccess
ISSN : 1999-4923
Fecha de publicación : 30-jun-2022
Centro : Universidad San Pablo-CEU
Aparece en las colecciones: Facultad de Farmacia





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