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Neuronal and astrocytic tetraploidy is increased in drug-resistant epilepsy.

Título : Neuronal and astrocytic tetraploidy is increased in drug-resistant epilepsy.
Autor : Sanz García, Ancor
Sánchez Jiménez, Patricia
Granero Cremades, Inmaculada
Toledo, María de
Pullido, Paloma
Navas, Marta
Frade, José María
Pereboom Maicas, Matilde Desirée
Torres Díaz, Cristina Virginia
Ovejero Benito, María del Carmen
Materias: Astrocytesflow cytometryneural cell cyclepolyploidyrefractory epilepsytemporal lobe epilepsytetraploidy
Editorial : Wiley
Citación : Sanz-García A, Sánchez-Jiménez P, Granero-Cremades I, et al. Neuronal and astrocytic tetraploidy is increased in drug-resistant epilepsy. Neuropathol Appl Neurobiol. 2023;49(1):e12873. doi:10.1111/nan.12873
Resumen : Aims: Epilepsy is one of the most prevalent neurological diseases. A third of patients with epilepsy remain drug-resistant. The exact aetiology of drug-resistant epilepsy (DRE) is still unknown. Neuronal tetraploidy has been associated with neuropathology. The aim of this study was to assess the presence of tetraploid neurons and astrocytes in DRE. Methods: For that purpose, cortex, hippocampus and amygdala samples were obtained from patients subjected to surgical resection of the epileptogenic zone. Post-mortem brain tissue of subjects without previous records of neurological, neurodegenerative or psychiatric diseases was used as control. Results: The percentage of tetraploid cells was measured by immunostaining of neurons (NeuN) or astrocytes (S100β) followed by flow cytometry analysis. The results were confirmed by image cytometry (ImageStream X Amnis System Cytometer) and with an alternative astrocyte biomarker (NDRG2). Statistical comparison was performed using univariate tests. A total of 22 patients and 10 controls were included. Tetraploid neurons and astrocytes were found both in healthy individuals and DRE patients in the three brain areas analysed: cortex, hippocampus and amygdala. DRE patients presented a higher number of tetraploid neurons (p = 0.020) and astrocytes (p = 0.002) in the hippocampus than controls. These results were validated by image cytometry. Conclusions: We demonstrated the presence of both tetraploid neurons and astrocytes in healthy subjects as well as increased levels of both cell populations in DRE patients. Herein, we describe for the first time the presence of tetraploid astrocytes in healthy subjects. Furthermore, these results provide new insights into epilepsy, opening new avenues for future treatment.
URI : http://hdl.handle.net/10637/14119
Derechos: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
openAccess
ISSN : 1365-2990 (Electrónico)
Cubierto por: Acuerdo Transformativo -2023
Fecha de publicación : 10-ene-2023
Centro : Universidad San Pablo-CEU
Aparece en las colecciones: Facultad de Farmacia





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