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In vitro and in vivo gastrointestinal survival of non-encapsulated and microencapsulated "Salmonella" bacteriophages : implications for bacteriophage therapy in poultry

Título : In vitro and in vivo gastrointestinal survival of non-encapsulated and microencapsulated "Salmonella" bacteriophages : implications for bacteriophage therapy in poultry
Autor : Lorenzo Rebenaque, Laura
Malik, Danish J.
Catalá Gregori, Pablo
Marín Orenga, Clara
Sevilla Navarro, Sandra
Materias: Aves de corral - Enfermedades.Poultry - Diseases.Bacteriófagos - Uso terapéutico.Salmonella - Control.Chickens - Diseases.Pollos - Enfermedades.Bacteriophages - Therapeutic use.
Editorial : MDPI
Citación : Lorenzo-Rebenaque, L., Malik, D.J., Catalá-Gregori, P., Marin, C. & Sevilla-Navarro, S. (2021). In vitro and in vivo gastrointestinal survival of non-encapsulated and microencapsulated "Salmonella" bacteriophages: implications for bacteriophage therapy in poultry. Pharmaceuticals, vol. 14, i. 5 (06 may.), art. 434. DOI: https://doi.org/10.3390/ph14050434
Resumen : The therapeutic use of bacteriophages is recognized as a viable method to control Salmonella. Microencapsulation of phages in oral dosage forms may protect phages from inherent challenges of the gastrointestinal tract in chickens. Therefore, the main objective of this study was to assess the survival of Salmonella BP FGS011 (non-encapsulated and microencapsulated) through the gastrointestinal tract under in vitro as well as in vivo conditions after oral administration to 1-day-old chicks. To this end, the phage FGS011 was encapsulated in two different pH-responsive formulations with polymers Eudragit® L100, and Eudragit® S100 using the process of spray drying. Phages encapsulated in either of the two formulations were able to survive exposure to the proventriculus-gizzard in vitro conditions whereas free phages did not. Moreover, phages formulated in polymer Eudragit® S100 would be better suited to deliver phage to the caeca in chickens. In the in vivo assay, no statistically significant differences were observed in the phage concentrations across the gastrointestinal tract for either the free phage or the encapsulated phage given to chicks. This suggested that the pH of the proventriculus/gizzard in young chicks is not sufficiently acidic to cause differential phage titre reductions, thereby allowing free phage survival in vivo.
Descripción : Este artículo se encuentra disponible en la siguiente URL: https://www.mdpi.com/1424-8247/14/5/434
Este artículo pertenece al número especial "Bacteriophages as Therapeutic Delivery Vehicles".
URI : http://hdl.handle.net/10637/13976
Derechos: http://creativecommons.org/licenses/by/4.0/deed.es
ISSN : 1424-8247 (Electrónico)
Fecha de publicación : 6-may-2021
Centro : Universidad Cardenal Herrera-CEU
Aparece en las colecciones: Dpto. Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos





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