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dc.contributor.otherProducción Científica UCH 2019-
dc.contributor.otherUCH. Departamento de Medicina y Cirugía Animal-
dc.creatorBarranco Benacloch, Tomás-
dc.creatorPeres Rubio, Camila-
dc.creatorTvarijonaviciute, Asta-
dc.creatorRubio Zaragoza, Mónica.-
dc.creatorDamiá Giménez, Elena-
dc.creatorLamy, Elsa-
dc.creatorCugat Bertomeu, Ramón-
dc.creatorCerón Madrigal, José Joaquín-
dc.creatorTecles, Fernando-
dc.creatorEscribano Tortosa, Damián-
dc.date2019-
dc.date.accessioned2019-12-17T05:00:11Z-
dc.date.available2019-12-17T05:00:11Z-
dc.date.issued2019-02-15-
dc.identifier.citationBarranco, T., Rubio, CP., Tvarijonaviciute, A., Rubio, M., Damia, E., Lamy, E. et al. (2019). Changes of salivary biomarkers under different storage conditions : peffects of temperature and length of storage. Biochemia Medica, vol. 29, n. 1 (15 feb.), art. 010706. DOI: https://doi.org/10.11613/BM.2019.010706-
dc.identifier.issn1330-0962-
dc.identifier.issn1846-7482 (Electrónico)-
dc.identifier.urihttp://hdl.handle.net/10637/10760-
dc.descriptionEste artículo se ha publicado de forma definitiva en: https://www.biochemia-medica.com/en/journal/29/1/10.11613/BM.2019.010706-
dc.descriptionEn este artículo también participan: Camila P Rubio, Asta Tvarijonaviciute, Mónica Rubio, Elena Damia, Elsa Lamy, Ramón Cugat, José J. Cerón, Fernando Tecles y Damián Escribano.-
dc.description.abstractIntroduction: In this report, we aimed to examine the stability of various analytes in saliva under different storage conditions. Materials and methods: Alpha-amylase (AMY), cholinesterase (CHE), lipase (Lip), total esterase (TEA), creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LD), lactate (Lact), adenosine deaminase (ADA), Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability (FRAS), cupric reducing antioxidant capacity (CUPRAC), uric acid (UA), catalase (CAT), advanced oxidation protein products (AOPP) and hydrogen peroxide (H2O2) were colorimetrically measured in saliva obtained by passive drool from 12 healthy voluntary donors at baseline and after 3, 6, 24, 72 hours, 7 and 14 days at room temperature (RT) and 4 ºC, and after 14 days, 1, 3 and 6 months at – 20 ºC and – 80 ºC. Results: At RT, changes appeared at 6 hours for TEA and H2O2; 24 hours for Lip, CK, ADA and CUPRAC; and 72 hours for LD, Lact, FRAS, UA and AOPP. At 4 ºC changes were observed after 6 hours for TEA and H2O2; 24 hours for Lip and CUPRAC; 72 hours for CK; and 7 days for LD, FRAS and UA. At – 20ºC changes appeared after 14 days for AST, Lip, CK and LD; and 3 months for TEA and H2O2. At – 80 ºC observed changes were after 3 months for TEA and H2O2. Conclusions: In short-term storage, the analytes were more stable at 4 ºC than at room temperature, whereas in long-term storage they were more stable at - 80 ºC than at – 20 ºC.-
dc.formatapplication/pdf-
dc.language.isoen-
dc.publisherCroatian Society of Medical Biochemistry.-
dc.relationEste artículo ha recibido financiación de la Fundación Garcia Cugat y de la Fundación Seneca de la Región de Murcia.-
dc.relation.ispartofBiochemia Medica, vol. 29, n. 1 (15 feb. 2019).-
dc.rightshttp://creativecommons.org/licenses/by/4.0/deed.es-
dc.subjectEstrés oxidativo.-
dc.subjectOxidative stress.-
dc.subjectMarcadores bioquímicos.-
dc.subjectSaliva - Análisis.-
dc.subjectBiochemical markers.-
dc.subjectSaliva - Analysis.-
dc.subjectEnzimas - Análisis.-
dc.subjectEnzymes - Analysis.-
dc.titleChanges of salivary biomarkers under different storage conditions : peffects of temperature and length of storage-
dc.typeArtículo-
dc.identifier.doihttps://doi.org/10.11613/BM.2019.010706-
dc.centroUniversidad Cardenal Herrera-CEU-
Aparece en las colecciones: Dpto. Medicina y Cirugía Animal




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