Please use this identifier to cite or link to this item: http://hdl.handle.net/10637/10608

Organotypic retinal explant cultures as in vitro alternative for diabetic retinopathy studies


Thumbnail

See/Open:
 Organotypic_Valdes_ALTEX_2016.pdf
972,38 kB
Adobe PDF
Title: Organotypic retinal explant cultures as in vitro alternative for diabetic retinopathy studies
Authors : Valdés, Joaquín
Trachsel Moncho, Laura
Sahaboglu, Ayse
Trifunović, Dragana
Miranda Sanz, María
Ueffing, Marius
Paquet-Durand, François
Schmachtenberg, Oliver
Keywords: Diabetes - Complicaciones y secuelas.Muerte celular.Diabetic retinopathy.Diabetes - Complications.Retinopatía diabética.Cell death.
Publisher: Springer
Citation: Valdés, J., Trachsel-Moncho, L., Sahaboglu, A., Trifunović, D., Miranda, M., Ueffing, M. et al. (2016). Organotypic retinal explant cultures as in vitro alternative for diabetic retinopathy studies. ALTEX : alternatives to animal experimentation, vol. 33, n. 4 (9 may. 2016), pp. 459-464. DOI: https://doi.org/10.14573/altex.1603111
Abstract: Diabetic retinopathy (DR) is a major cause of vision loss and one of the most common and debilitating complications of diabetes. Research to prevent DR is hindered by a lack of experimental model systems that faithfully reproduce the disease pathology, in particular for type 2 diabetes, which requires prolonged disease progression in animals to develop some hallmarks of DR. Here, we introduce an alternative in vitro model system for DR, based on serum-free, organotypic rodent retinal explant cultures, which allow physiological and pharmacological manipulation of the retina for up to two weeks under tightly controlled conditions. Retinal explant cultures have the advantage of isolating direct neuronal consequences of diabetic conditions from indirect systemic effects mediated via the retinal vasculature or the immune system. Exposed to conditions emulating type 1 or type 2 diabetes, retinal explants displayed elevated cell death rates among inner retinal neurons as well as photoreceptors, with a particularly strong loss of cone photoreceptors. Our results support a direct impact of diabetic conditions on retinal neurons and may help explain color vision defects observed in DR patients. This serum-free in vitro DR model avoids the animal suffering of established DR models and reduces the overall number of animals needed for such research. It should prove useful to study the mechanisms of neuronal cell death caused by DR and to screen for potential future DR treatments.
Description: Este artículo se encuentra disponible en la siguiente URL: https://www.altex.org/index.php/altex/article/view/144
URI: http://hdl.handle.net/10637/10608
Rights : http://creativecommons.org/licenses/by/4.0/deed.es
ISSN: 1868-596X
1868-8551 (Electrónico)
Issue Date: 9-May-2016
Center : Universidad Cardenal Herrera-CEU
Appears in Collections:Dpto. Ciencias Biomédicas





Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.