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dc.creatorBarbas Arribas, Coral.-
dc.creatorCastro, Mario-
dc.creatorBonet Serra, Bartolomé-
dc.creatorViana Arribas, Marta-
dc.creatorHerrera Castillón, Emilio.-
dc.date1997-
dc.date.accessioned2011-09-19T15:40:16Z-
dc.date.available2011-09-19T15:40:16Z-
dc.date.issued1997-
dc.identifier000000406677-
dc.identifier.urihttp://hdl.handle.net/10637/1017-
dc.descriptionEn: Journal of chromatography A, ISSN 0021-9673 1997. n. 778 : 415-420 p.-
dc.description.abstractA high-performance liquid chromatographic (HPLC) method to determine vitamins A, Ap and E simultaneously was developed with direct extraction of vitamins from rat tissues with n-hexane and probe sonicating. The dry residue was redissolved in chloroform-methanol. Vitamins A and Ap were detected by UV-Vis and vitamin Eby fluorescence. Vitamin K, used as internal standard, was detected both by UV-Vis and by fluorescence. Standards and samples were checked for linearity giving correlation coefficients that were higher than 0.99 in the concentration range of 3.1-9.4 for vitamin A, 8.2-24.7 for vitamin Ap and 0.6-1.7 nmol/g in the case of liver extracts and 0.5-3.0 nmol/g in the case of placenta. The intra-assay precision (R.S.D.) varied between 1.48 and 7.25, whereas inter-assay precision was between 4.99 and 7.03. Recoveries ranged between 94±8 and 107±5%. Results from the application of this method to different rat tissues having wide range of vitamin content are presented.en-EN
dc.formatapplication/pdf-
dc.language.isoen-
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.es-
dc.subjectVitaminsen-EN
dc.titleSimultaneous determination of vitamins A and E in rat tissues by high-performance liquid chromatography.-
dc.typeArtículo-
europeana.dataProviderUNIVERSIDAD SAN PABLO CEU-
europeana.isShownAthttp://hdl.handle.net/10637/1017-
europeana.objecthttp://repositorioinstitucional.ceu.es/visor/libros/406677/thumb_europeana/406677.jpg-
europeana.providerHispana-
europeana.rightshttp://creativecommons.org/publicdomain/zero/1.0/-
europeana.typeTEXT-
dc.centroUniversidad San Pablo-CEU-
Aparece en las colecciones: Facultad de Farmacia




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