Please use this identifier to cite or link to this item: http://hdl.handle.net/10637/15427
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dc.contributor.otherUniversidad San Pablo-CEU. Facultad de Medicina.-
dc.contributor.otherGrupo: Centro de metabolómica y bioanálisis (CEMBIO)-
dc.creatorFernández García, Miguel-
dc.creatorAres-Arroyo, Manuel-
dc.creatorWedel, Emilia-
dc.creatorMontero, Natalia-
dc.creatorBarbas Arribas, Coral.-
dc.creatorRey-Stolle, María Fernanda-
dc.creatorGonzález Zorn, Bruno-
dc.creatorGarcía Fernández, Antonia-
dc.date.accessioned2024-02-09T15:15:26Z-
dc.date.available2024-02-09T15:15:26Z-
dc.date.issued2023-07-06-
dc.identifier.citationFernández-García M, Ares-Arroyo M, Wedel E, Montero N, Barbas C, Rey-Stolle MF, González-Zorn B, García A. Multiplatform Metabolomics Characterization Reveals Novel Metabolites and Phospholipid Compositional Rules of Haemophilus influenzae Rd KW20. Int J Mol Sci. 2023 Jul 6;24(13):11150. doi: 10.3390/ijms241311150. PMID: 37446331; PMCID: PMC10342370.es_ES
dc.identifier.issn1422-0067-
dc.identifier.urihttp://hdl.handle.net/10637/15427-
dc.description.abstractHaemophilus influenzae is a gram-negative bacterium of relevant clinical interest. H. influenzae Rd KW20 was the first organism to be sequenced and for which a genome-scale metabolic model (GEM) was developed. However, current H. influenzae GEMs are unable to capture several aspects of metabolome nature related to metabolite pools. To directly and comprehensively characterize the endometabolome of H. influenzae Rd KW20, we performed a multiplatform MS-based metabolomics approach combining LC-MS, GC-MS and CE-MS. We obtained direct evidence of 15-20% of the endometabolome present in current H. influenzae GEMs and showed that polar metabolite pools are interconnected through correlating metabolite islands. Notably, we obtained high-quality evidence of 18 metabolites not previously included in H. influenzae GEMs, including the antimicrobial metabolite cyclo(Leu-Pro). Additionally, we comprehensively characterized and evaluated the quantitative composition of the phospholipidome of H. influenzae, revealing that the fatty acyl chain composition is largely independent of the lipid class, as well as that the probability distribution of phospholipids is mostly related to the conditional probability distribution of individual acyl chains. This finding enabled us to provide a rationale for the observed phospholipid profiles and estimate the abundance of low-level species, permitting the expansion of the phospholipidome characterization through predictive probabilistic modelling.en_EN
dc.formatapplication/pdf-
dc.language.isoen-
dc.publisherMDPI-
dc.relation.ispartofInternational Journal of Molecular Sciences-
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.es-
dc.subjectHaemophilus influenzaeen_EN
dc.subjectLipidome characterizationen_EN
dc.subjectMetabolome characterizationen_EN
dc.subjectMicrobial lipidomicsen_EN
dc.subjectMicrobial metabolomicsen_EN
dc.subjectPhospholipid compositional modelen_EN
dc.subjectPhospholipidomeen_EN
dc.titleMultiplatform Metabolomics Characterization Reveals Novel Metabolites and Phospholipid Compositional Rules of Haemophilus influenzae Rd KW20en_EN
dc.typeArtículoes_ES
dc.identifier.doi10.3390/ijms241311150-
dc.relation.projectIDFunded by the Ministry of Science and Innovation of Spain, grant number PID2021-122490NB-I00/AEI/10.13039/501100011033, and cofunded by the European Regional Development Fund ERDF, “A way of making Europe”. The APC was funded by the European Regional Development Fund ERDF, “A way of making Europe”, grant number PID2021-122490NB-I00.-
dc.centroUniversidad San Pablo-CEU-
Appears in Collections:Medicina




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