Please use this identifier to cite or link to this item: http://hdl.handle.net/10637/15418
Full metadata record
DC FieldValueLanguage
dc.contributor.otherUniversidad San Pablo-CEU. Facultad de Medicina-
dc.contributor.otherGrupo: Respuesta inmune en la Esclerosis Múltiple (RIEM)-
dc.creatorMagnet, Angela-
dc.creatorGomes, Thiago Santos-
dc.creatorPardinas, Carmen-
dc.creatorGarcía de Blas, Natalia-
dc.creatorSádaba Argaiz, María Cruz-
dc.creatorCarrillo, Eugenia-
dc.creatorIzquierdo Arias, Fernando-
dc.creatorBenítez del Castillo, José Manuel-
dc.creatorHurtado Marcos, Carolina-
dc.creatorÁguila de la Puente, Carmen del-
dc.creatorFenoy Rodríguez, Soledad-
dc.date.accessioned2024-02-09T12:34:36Z-
dc.date.available2024-02-09T12:34:36Z-
dc.date.issued2018-01-22-
dc.identifier.citationMagnet, A., Gomes, T.S., Pardinas, C. et al. Can artificial tears prevent Acanthamoeba keratitis? An in vitro approach. Parasites Vectors 11, 50 (2018). https://doi.org/10.1186/s13071-018-2639-5-
dc.identifier.issn1756-3305-
dc.identifier.urihttp://hdl.handle.net/10637/15418-
dc.description.abstractBackground: The use of contact lenses has increased in recent years as has the incidence of Dry Eye Syndrome, partly due to their use. Artificial tears are the most common treatment option. Since these changes can facilitate Acanthamoeba infection, the present study has been designed to evaluate the effect of three artificial tears treatments in the viability of Acanthamoeba genotype T4 trophozoites. Optava Fusion™, Oculotect®, and Artelac® Splash were selected due to their formulation. Methods: Viability was assessed using two staining methods, Trypan Blue stain and CTC stain at different time intervals (2, 4, 6, 8 and 24 h). Trypan Blue viability was obtained by manual count with light microscopy while the CTC stain was determined using flow cytometry. Results: Trypan Blue staining results demonstrated a decrease in viability for Optava Fusion™ and Artelac® Splash during the first 4 h of incubation. After, this effect seems to lose strength. In the case of Oculotect®, complete cell death was observed after 2 h. Using flow cytometry analysis, Optava Fusion™ and Oculotect® exhibited the same effect observed with Trypan Blue staining. However, Artelac® Splash revealed decreasing cell respiratory activity after four hours, with no damage to the cell membrane. Conclusions: The present study uses, for the first time, CTC stain analyzed by flow cytometry to establish Acanthamoeba viability demonstrating its usefulness and complementarity with the traditional stain, Trypan Blue. Artelac® Splash, with no preservatives, and Optava Fusion TM, with Purite®, have not shown any useful amoebicidal activity. On the contrary, promising results presented by Ocultect®, with BAK, open up a new possibility for Acanthamoeba keratitis prophylaxis and treatment although in vivo studies should be carried out.en_EN
dc.formatapplication/pdf-
dc.language.isoen-
dc.publisherBMC-
dc.relation.ispartofParasites and Vectors-
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/deed.es-
dc.subjectAcanthamoebaen_EN
dc.subjectProphylaxisen_EN
dc.subjectArtificial tearsen_EN
dc.subjectPreservativesen_EN
dc.subjectAmoebicidal effecten_EN
dc.titleCan artificial tears prevent Acanthamoeba keratitis? An in vitro approachen_EN
dc.typeArtículoes_ES
dc.identifier.doi10.1186/s13071-018-2639-5-
dc.relation.projectIDGrant PI12/ 02725 from Instituto de Salud Carlos III (FISS) and FEDER-
dc.relation.projectIDgrant USPCEU-PC07/2013 of the Fundación Universitaria San Pablo CEU-
dc.relation.projectIDCAPES/Brazil (Grant BEX 9132/13-9)-
dc.centroUniversidad San Pablo-CEU-
Appears in Collections:Medicina




Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.