Analytical approaches for studying oxygenated lipids in the search of potential biomarkers by LC-MS.

dc.centroUniversidad San Pablo-CEU
dc.contributor.authorBarbas Arribas, Coral
dc.contributor.authorVillaseñor Solis, Alma Cristina
dc.contributor.authorGonzález Riaño, Carolina
dc.contributor.authorBarker Tejeda, Tomas Clive
dc.contributor.authorGodzien, Joanna Barbara
dc.contributor.authorLópez López, Ángeles
dc.contributor.authorDudzik, Danuta
dc.contributor.authorGradillas Nicolás, Ana
dc.contributor.otherUniversidad San Pablo-CEU. Facultad de Medicina
dc.date2021
dc.date.accessioned2021-09-16T04:00:13Z
dc.date.available2021-09-16T04:00:13Z
dc.date.issued2021-09-16
dc.descriptionEn: TrAC Trends in Analytical Chemistry. ISSN 0165-9936 2021, v. 143, 116367
dc.description.abstractGreat advances in lipidomics during the last years have opened the door to a broader knowledge of oxygenated lipids. These substances are derived either from the inclusion of previously hydroxylated fatty acids in the lipid structure of sphingolipids and acyl-L-carnitines, or by enzymatic and nonenzymatic modifications (oxidized lipids) of glycerophospholipids (including cardiolipins), cholesteryl esters and cholesterol. Despite their significance in the regulation of multiple diseases such as cancer or diabetes, the number of experimentally detected oxygenated lipids remains relatively low. This is in part due to the main challenges in their analysis, which are their low natural concentrations, their wide diversity of physicochemical properties, presence of isomers, and their a priori unknown presence in the biological samples. In particular, analysis of oxidized lipids, especially peroxides, has become a daunting task in liquid chromatography coupled to mass spectrometry (LC-MS) due to their high chemical and thermal instability, and the potential for further propagation of lipid oxidation and eventual degradation. The aim of this review is to highlight the experimental conditions on sample preparation procedures, the LC-MS based analytical approaches for identification and quantification of oxygenated lipids, and their relation as potential biomarkers in diseases based on the most relevant articles published in the last five years. Regarding sample preparation, special attention has been given to antioxidants, internal standards, extraction and concentration methods, and derivatization approaches. Moreover, targeted, semi-targeted and non-targeted strategies have been discussed presenting examples. Finally, considerations on the structural identification, one of the main challenges, are presented.en-EN
dc.formatapplication/pdf
dc.identifier000000723574
dc.identifier.urihttp://hdl.handle.net/10637/13005
dc.language.isoen
dc.relationThis work was supported by Ministry of Science, Innovation and Universities of Spain (Ref. RTI2018-095166-B-I00) and “la Caixa” Foundation (Ref. 01-09-2018-01-09-2020). T.C.B-T and A.L.L. thank CEU-International Doctoral School (CEINDO) for their fellowship.en-EN
dc.rightsopen access
dc.rights.cchttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subjectOxidized lipidsen-EN
dc.subjectBiomarkers LC-MS analysisen-EN
dc.subjectAnnotationen-EN
dc.subjectLipidomicsen-EN
dc.subjectOxysterolsen-EN
dc.subjectOxidized phospholipidsen-EN
dc.subjectSphingolipidsen-EN
dc.subjectAcylcarnitinesen-EN
dc.subjectOxidized cholesteryl estersen-EN
dc.titleAnalytical approaches for studying oxygenated lipids in the search of potential biomarkers by LC-MS.
dc.typeArtículo
dspace.entity.typePublicationes
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