Here, we describe a 3-month-old XX male French Bulldog.The diagnosis was based on the clinical signs, gonadalhistology and cytogenetic analysis. Additionally, the dog wasconfirmed to be Sry negative by semi-quantitative reversetranscription polymerase chain reaction (sqRT-PCR). CanineSry-negative XX sex reversal is a disorder of gonadaldevelopment where individuals who have a female karyotypedevelop testes or ovotestes. To our knowledge, this case is thefirst XX male sex reversion described in a French Bulldog.