Probanza Lobo, Agustín

Influenza A virus (IAV) infection triggers quantitative and qualitative modifications in lung and intestinal microbiota composition, which contain an important reservoir of antibiotic resistance genes. Analysis of genetic changes is a common practice in studies that analyze microbiota modifications. However, there is little evidence of functional changes linked to such microbiota modifications. This study evaluates some cecal microbiota’s functional changes, comparing sublethal IAV with mock-infected mice. Community-wide phenotypic metabolic profile (Biolog EcoPlatesTM) and relative antibiotic resistance changes to clinically relevant antibiotics (cenoantibiogram) have been performed in this context. Results reveal a temporal association between IAV infection and alterations in nutrient substrate profile usage as well as changes in antibiotic resistance of cecal microbiota. Alterations are transient and predominantly occur at early time points post-IAV infection. There is a functional rebalance in nutrient substrate usage and antibiotic resistance under the established culture conditions, accompanied by a decrease in microbial density of the cecal community on days 5 and 7 after the IAV infection. Our data underline that active IAV infections altering microbial populations are associated with changes in nutrient usage preferences and affectaffectaffectcommunity behaviors toward specific antibiotics. These findings could have implications including activation of nutrient-related metabolic stress at the microbiota community level and additional antibiotic resistance selection mechanisms of clinically relevant infections.

Antecedentes. En un contexto marcado por el modelo de aprendizaje colaborativo y constructivista y la rápida evolución de las tecnologías de la información y las comunicaciones, los equipos de gobierno de las universidades deben apoyar decididamente las iniciativas del profesorado en materia de innovación docente y promover acciones de impulso complementarias. Método. Uno de los objetivos de nuestro Vicerrectorado de Profesorado e Investigación es promover la innovación docente mediante el apoyo a la elaboración de un programa formativo adecuado para el profesorado; la convocatoria de premios de innovación docente; el apoyo a la celebración anual de congresos interfacultativos y la programación de talleres internos impartidos por los propios profesores. Resultados. Tras una participación creciente en todos los ámbitos a lo largo de los últimos años, durante el curso 2019-2020 nuestros profesores (alrededor de 1000) cursaron 7958 horas de formación y presentaron 21 proyectos a la convocatoria de premios de innovación docente y 103 comunicaciones al Congreso CEU de Innovación Educativa y Docente. Además, participaron en un número muy elevado de actividades adicionales de transformación digital, una situación extraordinaria impuesta por la pandemia. Conclusiones. Los resultados obtenidos avalan la pertinencia de las iniciativas emprendidas y recomiendan su mantenimiento en el futuro.

A screening often different Mediterranean crops was carried out to test their tolerance to soils polluted with copper and zinc. The methods for copper and zinc determination in plants have been improved, avoiding the time consuming acid digestion. Results showed that zinc toxicity was higher than copper for the assayed plants. In copper-polluted soils, Lupinus luteus (lupin) 549µgg- 1 and Zea mays (corn) 213µgg- 1 showed the highest amounts dried weight of accumulated metal, mainly detected associated to the root system without significant reduction of biometrical parameters of the plants. In zinccontaminated soils, corn (1304 µgg- 1 ), Triticum durum (wheat) (933 µgg- 1 ) and lupin (654µgg- 1 ) showed the highest amounts of metal accumulated mainly in the roots, and in all cases, without biometrical reduction.