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Collagen cross-linking lignin improves the bonding performance of etch-and-rinse adhesives to dentin


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Title: Collagen cross-linking lignin improves the bonding performance of etch-and-rinse adhesives to dentin
Authors : Paula, Diego M. de
Lomonaco, Diego
Parente da Ponte, Antonio Moisés
Cordeiro, Karen Evellin
Moreira, Madiana Magalhaes
Giovarruscio, Massimo
Sauro, Salvatore.
Feitosa, Victor Pinheiro
Keywords: Dental materials.Materiales dentales.Materiales biomédicos.Dentina.Dental adhesives.Adhesivos dentales.Dentin.Biomedical materials.
Publisher: MDPI
Citation: de Paula, D. M., Lomonaco, D., Parente da Ponte, A. M., Cordeiro, K. E., Magalhães Moreira, M., Giovarruscio, M., Sauro, S., & Pinheiro Feitosa, V. (2022). Collagen cross-linking lignin improves the bonding performance of etch-and-rinse adhesives to dentin. Materials, vol. 15, i. 9 (29 apr.), art. 3218. DOI: https://doi.org/10.3390/ma15093218
Abstract: To evaluate the biomodification ability of lignin used as pre-treatment in human dentin before the application of an etch-and-rinse adhesive. Experimental hydroethanolic solutions with different cross-linking agents were used: 6.5% proanthocyanidins (PAC, from grape-seed extract); 2% cardanol (CARD, from cashew-nut shell liquid); lignin (LIG, from eucalyptus) at 1, 2 or 4% concentrations. The negative control (NC) was ethanol 50 v%. Extracted molars were prepared, and dentin microtensile bond strength ( TBS) was evaluated after 24 h water storage or 10,000 thermocycling aging. Further specimens were processed for SEM nanoleakage, micropermeability confocal microscopy evaluation and in situ degree of conversion (DC) through micro-Raman spectroscopy. Demineralized dentin sticks were submitted to a three-point bending test to evaluate the elastic modulus (E) before and after 1 min biomodification using the tested solutions. Moreover, it was also evaluated the mass changes and hydroxyproline (HYP) release after 4-weeks of water storage. Vibrational collagen crosslinking identification was evaluated through micro-Raman spectroscopy. The results were analyzed by analysis of variance (ANOVA) and Tukey’s test ( = 0.05). A significant reduction in TBS was observed in groups NC (p < 0.001) and CARD (p = 0.026). LIG-4% showed no significant reduction in TBS after aging (p = 0.022). Nanoleakage micrographs showed hybrid layer protection with all agents, but reduced micropermeability was attained only with lignin. Polymerization was negatively affected in the presence of all tested cross-linking agents, except LIG-1%. Lignin and cardanol increased the dentin E values, but only lignin reduced the mass loss in dentin specimens. Effective collagen crosslinking (1117 cm􀀀1 and 1235 cm􀀀1) was detected for all agents. HYP release was significantly lower with LIG-1% than NC (p < 0.001). Lignin was able to perform collagen cross-linking and prevent the degradation of unprotected dentin collagen, thereby improving the bonding performance of the composite restorations performed in this study.
Description: Este artículo se encuentra disponible en la siguiente URL: https://www.mdpi.com/1996-1944/15/9/3218
Este artículo de investigación pertenece al número especial "Biomaterials in Conservative Dentistry".
URI: http://hdl.handle.net/10637/14360
Rights : http://creativecommons.org/licenses/by/4.0/deed.es
ISSN: 1996-1944 (Electrónico)
Language: es
Issue Date: 29-Apr-2022
Center : Universidad Cardenal Herrera-CEU
Appears in Collections:Dpto. Odontología





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